Proteintech, 33468-1-AP, FAT4 Polyclonal antibody

Size: 20ul / 150ul The FAT4 (33468-1-AP) by Proteintech is a Polyclonal antibody targeting FAT4 in WB, IHC, ELISA applications with reactivity to human samples 33468-1-AP targets FAT4 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HGC-27 cells, HUVEC cells, MDA-MB-231 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information FAT4 is a large transmembrane protein with a mass of approximately 530 kDa. It belongs to the cadherin superfamily and contains multiple cadherin repeats EGF-like domains and one laminin G-like domain. FAT4 is expressed widely across tissues with higher expression observed in the kidney liver and cardiovascular system indicating its involvement in diverse physiological processes. This protein plays a role in maintaining cell adhesion and communication. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag37862 Product name: Recombinant human FAT4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 4200-4504 aa of NM_001291285 Sequence: SVTPDTALSLEGKGRLDYHMSQNEKREYLLRQSLRGAMLEPFGVNSLEVKFRTRSENGVLIHIQESSNYTTVKIKNGKVYFTSDAGIAGKVERNIPEVYVADGHWHTFLIGKNGTATVLSVDRIYNRDIIHPTQDFGGLDVLTISLGGIPPNQAHRDAQTAGFDGCIASMWYGGESLPFSGKHSLASISKTDPSVKIGCRGPNICASNPCWGDLLCINQWYAYRCVPPGDCASHPCQNGGSCEPGLHSGFTCSCPDSHTGRTCEMVVACLGVLCPQGKVCKAGSPAGHVCVLSQGPEEISLPLWA Predict reactive species Full Name: FAT tumor suppressor homolog 4 (Drosophila) Calculated Molecular Weight: 4981aa, 543 kDa Observed Molecular Weight: 540 kDa GenBank Accession Number: NM_001291285 Gene Symbol: FAT4 Gene ID (NCBI): 79633 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q6V0I7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.