Proteintech, 51025-2-Ig, MECR Polyclonal antibody

Size: 20ul / 150ul The MECR (51025-2-Ig) by Proteintech is a Polyclonal antibody targeting MECR in WB, IHC, ELISA applications with reactivity to mouse samples 51025-2-Ig targets MECR in WB, IHC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Mitochondrial Trans-2-Enoyl-CoA Reductase is abbrivated as MECR, involved in human mitochonrial fatty acid synthesis(mtFAS) as an oxidoreductase. (PMID: 27817865) MECR has molecular weight can be calculated around 37KDa. With immunopercipitation reaction on mouse heart samples, the IgG heavy chain, dimer of MECR is around 65KDa. Numerous studies have shown disorder of lipid metabolism is closely related with malignance, especially in liver cancer. As futher research suggests that MECR has close realitionship with cell prolifereation, appotosis, and metastasis (PMID: 31178528) Specification Tested Reactivity: mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitochondrial trans-2-enoyl-CoA reductase Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC003864 Gene Symbol: MECR Gene ID (NCBI): 26922 RRID: AB_1167469 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9DCS3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.