Proteintech, 51064-2-AP, HA tag Polyclonal antibody

Size: 150ul The HA tag (51064-2-AP) by Proteintech is a Polyclonal antibody targeting HA tag in WB, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to recombinant protein samples 51064-2-AP targets HA tag in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: recombinant protein, Transfected HEK-293 cells Positive IP detected in: Transfected HEK-293 cells Positive IF/ICC detected in: Transfected HEK-293 cells Positive FC (Intra) detected in: Transfected HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag. Specification Tested Reactivity: recombinant protein Cited Reactivity: human, mouse, pig, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: HA tag Calculated Molecular Weight: 1 kDa RRID: AB_11042321 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.