Proteintech, 51070-2-AP, STAT4 Polyclonal antibody

Size: 20ul / 150ul The STAT4 (51070-2-AP) by Proteintech is a Polyclonal antibody targeting STAT4 in WB, ELISA applications with reactivity to human, mouse, rat samples 51070-2-AP targets STAT4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, mouse testis tissue, rat testis tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Background Information The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 4 Calculated Molecular Weight: 748 aa, 86 kDa Observed Molecular Weight: 86 kDa GenBank Accession Number: BC031212 Gene Symbol: STAT4 Gene ID (NCBI): 6775 RRID: AB_2286750 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14765 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.