Proteintech, 51085-1-AP, NFKB2 Polyclonal antibody

Size: 20ul / 150ul The NFKB2 (51085-1-AP) by Proteintech is a Polyclonal antibody targeting NFKB2 in WB, ELISA applications with reactivity to human, mouse samples 51085-1-AP targets NFKB2 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Apoptosised HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NFKB2. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) Calculated Molecular Weight: 97 kDa Observed Molecular Weight: 103 kDa GenBank Accession Number: BC002844 Gene Symbol: NFKB2 Gene ID (NCBI): 4791 ENSEMBL Gene ID: ENSG00000077150 RRID: AB_2267124 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q00653 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.