Proteintech, 51152-1-AP, ATP6V1A Polyclonal antibody

Size: 20ul / 150ul The ATP6V1A (51152-1-AP) by Proteintech is a Polyclonal antibody targeting ATP6V1A in WB, IHC, ELISA applications with reactivity to human, mouse samples 51152-1-AP targets ATP6V1A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, human brain tissue, mouse kidney tissue Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.This antibody is specific to ATP6V1A. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: NM_001690 Gene Symbol: ATP6V1A Gene ID (NCBI): 523 RRID: AB_2881254 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P38606 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.