Proteintech, 55018-1-AP, Annexin A1 Polyclonal antibody

Size: 20ul / 150ul The Annexin A1 (55018-1-AP) by Proteintech is a Polyclonal antibody targeting Annexin A1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 55018-1-AP targets Annexin A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: C2C12 cell, HeLa cells, NIH/3T3 cells, HepG2 cells, C6 cells, K-562 cells Positive IHC detected in: human colon tissue, human oesophagus tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes. The antibody is specific to ANXA1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, canine, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: annexin A1 Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: NM_000700 Gene Symbol: Annexin A1 Gene ID (NCBI): 301 RRID: AB_10860266 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04083 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.