Proteintech, 55069-1-AP, MYH2-specific Polyclonal antibody

Size: 20ul / 150ul The MYH2-specific (55069-1-AP) by Proteintech is a Polyclonal antibody targeting MYH2-specific in WB, IHC, ELISA applications with reactivity to human samples 55069-1-AP targets MYH2-specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: multi-cells/tissue, HSkMC cells, human skeletal muscle tissue Positive IHC detected in: human skeletal muscle tissue, human bladder tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody. Specification Tested Reactivity: human Cited Reactivity: mouse, rat, pig, canine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myosin, heavy chain 2, skeletal muscle, adult Calculated Molecular Weight: 223 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: NM_017534 Gene Symbol: MYH2 Gene ID (NCBI): 4620 RRID: AB_10860267 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UKX2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.