Proteintech, 55091-1-AP, PTCH2 Polyclonal antibody

Size: 20ul / 150ul The PTCH2 (55091-1-AP) by Proteintech is a Polyclonal antibody targeting PTCH2 in WB, ELISA applications with reactivity to human, mouse, rat samples 55091-1-AP targets PTCH2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, human brain tissue, mouse liver tissue, rat liver tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information PTCH1 (patched homolog 1) and PTCH2 are twelve-pass transmembrane proteins that act receptors of Hedgehog signaling pathway (PMID: 8906794; 9811851). Signals of Hedgehog family proteins (SHH, IHH and DHH in humans) are transduced through Patched family receptors (PTCH1 and PTCH2) and Smoothened (SMO) to GLI family transcription factors. Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). PTCH2 may function as a tumor suppressor in the Hedgehog signaling pathway. Alterations in PTCH2 gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. This antibody is specific to PTCH2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: patched homolog 2 (Drosophila) Calculated Molecular Weight: 131 kDa Observed Molecular Weight: 131-140 kDa GenBank Accession Number: NM_003738 Gene Symbol: PTCH2 Gene ID (NCBI): 8643 RRID: AB_10858392 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y6C5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.