Proteintech, 55106-1-AP, MEKK2 Polyclonal antibody

Size: 20ul / 150ul The MEKK2 (55106-1-AP) by Proteintech is a Polyclonal antibody targeting MEKK2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 55106-1-AP targets MEKK2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, MCF-7 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information MAP3K2, also named as MEKK2 and MEKK2B, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAPK kinase kinase subfamily. It is a component of a protein kinase signal transduction cascade. MAP3K2 mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. The antibody is specific to MAP3K2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase kinase kinase 2 Calculated Molecular Weight: 70 kDa Observed Molecular Weight: 65-75 kDa GenBank Accession Number: NM_006609 Gene Symbol: MAP3K2 Gene ID (NCBI): 10746 RRID: AB_11064604 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2U5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.