Proteintech, 55108-1-AP, ATP13A3 Polyclonal antibody

Size: 20ul / 150ul The ATP13A3 (55108-1-AP) by Proteintech is a Polyclonal antibody targeting ATP13A3 in WB, IHC, ELISA applications with reactivity to human samples 55108-1-AP targets ATP13A3 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: BxPC-3 cells, MCF-7 cells Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information ATP13A3 is a member of the P-type ATPase family of proteins that transport a variety of cations across membranes. ATP13A3 is a novel member of the enigmatic mammalian PTS that contributes to cellular polyamine uptake and is implicated in disease(PMID: 33310703). Mutations in ATP13A3 cause Primary Pulmonary Hypertension 5(PMID: 30679663, 34493544). ATP13A3 can be detected as 78 kDa representing a truncated isoform of ATP13A3 as reported (PMID: 35260637). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ATPase type 13A3 Calculated Molecular Weight: 138 kDa Observed Molecular Weight: 78 kDa GenBank Accession Number: NM_024524 Gene Symbol: ATP13A3 Gene ID (NCBI): 79572 RRID: AB_3670238 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H7F0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.