Proteintech, 55143-1-AP, PSMB11 Polyclonal antibody

Size: 20ul / 150ul The PSMB11 (55143-1-AP) by Proteintech is a Polyclonal antibody targeting PSMB11 in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples 55143-1-AP targets PSMB11 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells Positive IHC detected in: human thymus tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:200-1:600 Immunohistochemistry (IHC): IHC : 1:100-1:400 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: proteasome (prosome, macropain) subunit, beta type, 11 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 32-35 kDa GenBank Accession Number: NM_001099780 Gene Symbol: PSMB11 Gene ID (NCBI): 122706 RRID: AB_10863288 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: A5LHX3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.