Proteintech, 55182-1-AP, DCTN1/p150 glued Polyclonal antibody

Size: 20ul / 150ul The DCTN1/p150 glued (55182-1-AP) by Proteintech is a Polyclonal antibody targeting DCTN1/p150 glued in WB, IHC, IF/ICC, ELISA applications with reactivity to human, canine samples 55182-1-AP targets DCTN1/p150 glued in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples. Tested Applications Positive WB detected in: human brain tissue, HeLa cells, Jurkat cells Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MDCK cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1. Specification Tested Reactivity: human, canine Cited Reactivity: human, mussel Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: dynactin 1 (p150, glued homolog, Drosophila) Calculated Molecular Weight: 142 kDa Observed Molecular Weight: 135 kDa, 150 kDa GenBank Accession Number: NM_004082 Gene Symbol: p150 glued Gene ID (NCBI): 1639 RRID: AB_10837074 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14203 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.