Proteintech, 55187-1-AP, ATP1A1-Specific Polyclonal antibody

Size: 150ul The ATP1A1-Specific (55187-1-AP) by Proteintech is a Polyclonal antibody targeting ATP1A1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 55187-1-AP targets ATP1A1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: 37°C incubated mouse brain tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, Neuro-2a cells, MCF-7 cells Positive IHC detected in: human ovary cancer tissue, human colon tissue, human hepatocellular ca, human liver cancer tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells, Caco-2 cells Positive FC (Intra) detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide Calculated Molecular Weight: 113 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: NM_000701 Gene Symbol: ATP1A1 Gene ID (NCBI): 476 ENSEMBL Gene ID: ENSG00000163399 RRID: AB_10859261 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P05023 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.