Proteintech, 55191-1-AP, EWS Polyclonal antibody

Size: 20ul / 150ul The EWS (55191-1-AP) by Proteintech is a Polyclonal antibody targeting EWS in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 55191-1-AP targets EWS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, mouse testis tissue, Jurkat cells Positive IHC detected in: human colon cancer tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Ewing sarcoma breakpoint region 1 Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 68-70 kDa GenBank Accession Number: NM_005243 Gene Symbol: EWS Gene ID (NCBI): 2130 RRID: AB_10860907 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q01844 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.