Proteintech, 55192-1-AP, CSNK1A1 Polyclonal antibody

Size: 20ul / 150ul The CSNK1A1 (55192-1-AP) by Proteintech is a Polyclonal antibody targeting CSNK1A1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 55192-1-AP targets CSNK1A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse brain tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, U-87 MG cells, rat brain tissue Positive IHC detected in: human breast cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: casein kinase 1, alpha 1 Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 30-35 kDa, 42-45 kDa GenBank Accession Number: NM_001892 Gene Symbol: CSNK1A1 Gene ID (NCBI): 1452 RRID: AB_11183034 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P48729 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.