Proteintech, 55200-1-AP, MBD2 Polyclonal antibody

Size: 20ul / 150ul The MBD2 (55200-1-AP) by Proteintech is a Polyclonal antibody targeting MBD2 in WB, IHC, ELISA applications with reactivity to human samples 55200-1-AP targets MBD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, human brain tissue, A431 cells, HEK-293 cells Positive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: methyl-CpG binding domain protein 2 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 43-45 kDa GenBank Accession Number: NM_003927 Gene Symbol: MBD2 Gene ID (NCBI): 8932 RRID: AB_10852963 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UBB5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.