Proteintech, 55247-1-AP, MAP4K4 Polyclonal antibody

Size: 20ul / 150ul The MAP4K4 (55247-1-AP) by Proteintech is a Polyclonal antibody targeting MAP4K4 in WB, IHC, IP, ELISA applications with reactivity to human samples 55247-1-AP targets MAP4K4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HK-2 cells, Jurkat cells, PC-3 cells Positive IP detected in: Jurkat cells Positive IHC detected in: human skeletal muscle tissue, human placenta tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase kinase kinase kinase 4 Calculated Molecular Weight: 151 kDa Observed Molecular Weight: 130-160 kDa GenBank Accession Number: NM_004834 Gene Symbol: MAP4K4 Gene ID (NCBI): 9448 RRID: AB_10836939 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95819 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.