Proteintech, 55268-1-AP, Alpha Parvin/Actopaxin Polyclonal antibody

Size: 20ul / 150ul The Alpha Parvin/Actopaxin (55268-1-AP) by Proteintech is a Polyclonal antibody targeting Alpha Parvin/Actopaxin in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, canine samples 55268-1-AP targets Alpha Parvin/Actopaxin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples. Tested Applications Positive WB detected in: U2OS cells, HeLa cells, mouse kidney tissue Positive IP detected in: U2OS cells Positive IHC detected in: human stomach cancer tissue, human heart tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MDCK cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin. Specification Tested Reactivity: human, mouse, rat, canine Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: parvin, alpha Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: NM_018222 Gene Symbol: Alpha Parvin/PARVA Gene ID (NCBI): 55742 RRID: AB_10951112 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NVD7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.