Proteintech, 55270-1-AP, ZBTB33 Polyclonal antibody

Size: 20ul / 150ul The ZBTB33 (55270-1-AP) by Proteintech is a Polyclonal antibody targeting ZBTB33 in WB, IHC, IP, ELISA applications with reactivity to human samples 55270-1-AP targets ZBTB33 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells Positive IP detected in: A431 cells Positive IHC detected in: human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ZBTB33, also named as Transcriptional regulator Kaiso, is a 672 amino acid protein, which localizes in nucleus and cytoplasm. ZBTB33 as a transcriptional regulator with bimodal DNA-binding specificity binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters and may contribute to the repression of target genes of the Wnt signaling pathway. The calculated molecular weight of ZBTB33 is 75 kDa, but modified protein is about 100 kDa (PMID: 10207085 and PMID: 16354691) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: zinc finger and BTB domain containing 33 Calculated Molecular Weight: 74 kDa Observed Molecular Weight: 68-74 kDa GenBank Accession Number: NM_006777 Gene Symbol: ZBTB33 Gene ID (NCBI): 10009 RRID: AB_2881295 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86T24 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.