Proteintech, 55320-1-AP, SH2D5 Polyclonal antibody

Size: 20ul / 150ul The SH2D5 (55320-1-AP) by Proteintech is a Polyclonal antibody targeting SH2D5 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 55320-1-AP targets SH2D5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse kidney tissue Positive IHC detected in: human cerebellum tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SH2D5, or Src homology 2 domain-containing protein 5, is a mammalian-specific adaptor-like protein that contains an N-terminal phosphotyrosine-binding domain and a C-terminal Src homology 2 (SH2) domain. SH2D5 has been found to interact with the breakpoint cluster region protein, a RacGAP that is also highly expressed in the brain. This interaction occurs between the phosphotyrosine-binding domain of SH2D5 and an NxxF motif located within the N-terminal region of the breakpoint cluster region. Furthermore, SH2D5 has been implicated in the regulation of cell growth in hepatocellular carcinoma cells, where it binds to transketolase (TKT) and promotes the activity of STAT3, a key transcription factor involved in cell proliferation and survival. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SH2 domain containing 5 Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 43-47 kDa GenBank Accession Number: NM_001103160 Gene Symbol: SH2D5 Gene ID (NCBI): 400745 RRID: AB_2881307 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6ZV89 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.