Proteintech, 55333-1-AP, SEPN1 Polyclonal antibody

Size: 20ul / 150ul The SEPN1 (55333-1-AP) by Proteintech is a Polyclonal antibody targeting SEPN1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 55333-1-AP targets SEPN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, NIH/3T3 cells Positive IHC detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse uterus tissue, mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SEPN1 (Selenoprotein N, 1) is a selenoprotein that plays a crucial role in maintaining cellular redox homeostasis and calcium homeostasis. It is encoded by the SEPN1 gene and is widely expressed in various tissues, particularly in skeletal muscle. (PMID: 32661288) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: selenoprotein N, 1 Calculated Molecular Weight: 66 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: NM_020451 Gene Symbol: SEPN1 Gene ID (NCBI): 57190 RRID: AB_2881308 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NZV5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.