Proteintech, 55370-1-AP, SKOR2 Polyclonal antibody

Size: 20ul / 150ul The SKOR2 (55370-1-AP) by Proteintech is a Polyclonal antibody targeting SKOR2 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 55370-1-AP targets SKOR2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse cerebellum tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information SKOR2 (SKI family transcriptional corepressor 2), also named FUSSEL18, is a transcriptional repressor that is highly expressed in the central nervous system (PMID: 16200078). SKOR2 was detected at 30 and 60 kD in the cerebellum. It is identified as a novel transcriptional regulator in Purkinje cells that acts upstream of Shh during cerebellum development (PMID: 21937600). Skor2 was detected in Purkinje cells in the human fetal cerebellum (PMID: 34140698). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: functional smad suppressing element 18 Calculated Molecular Weight: 104 kDa Observed Molecular Weight: 30kDa, 60 kDa GenBank Accession Number: XM_001715219 Gene Symbol: SKOR2 Gene ID (NCBI): 652991 RRID: AB_3086446 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q2VWA4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.