Proteintech, 55426-1-AP, TSN Polyclonal antibody

Size: 20ul / 150ul The TSN (55426-1-AP) by Proteintech is a Polyclonal antibody targeting TSN in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 55426-1-AP targets TSN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, MCF-7 cells Positive IP detected in: MCF-7 cells Positive IHC detected in: mouse testis tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TSN, also named as Translin or Component 3 of promoter of RISC, is a 228 amino acid protein, which belongs to the translin family. TSN as a DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translations, mostly is involved in immunoglobulin T-cell receptor gene segments. TSN May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: translin Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: NM_004622 Gene Symbol: TSN Gene ID (NCBI): 7247 RRID: AB_2881326 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15631 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.