Proteintech, 55493-1-AP, MAML1 Polyclonal antibody

Size: 20ul / 150ul The MAML1 (55493-1-AP) by Proteintech is a Polyclonal antibody targeting MAML1 in WB, ELISA applications with reactivity to human, mouse, rat samples 55493-1-AP targets MAML1 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse heart tissue, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mastermind-like 1 (Drosophila) Calculated Molecular Weight: 108 kDa Observed Molecular Weight: 108-120 kDa GenBank Accession Number: NM_014757 Gene Symbol: MAML1 Gene ID (NCBI): 9794 RRID: AB_11182286 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92585 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.