Proteintech, 60004-1-Ig, GAPDH Monoclonal antibody

Size: 150ul The GAPDH (60004-1-Ig) by Proteintech is a Monoclonal antibody targeting GAPDH in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat, pig, zebrafish, yeast samples 60004-1-Ig targets GAPDH in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish, yeast samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast, whole Nematode tissue, soybean whole plant tissue, arabidopsis whole plant tissue, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, C6 cells, PC-12 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue Positive IP detected in: HeLa Cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:50000-1:500000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:3000-1:16000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information What is the molecular weight of GAPDH?The molecular weight of GAPDH is 36 kD.What is GAPDH?GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping"protein.It is recognizedas an important enzyme involved in metabolic pathways, aiding in the glycolyticproduction of ATPand pyruvate (PMID: 21895736).What is the function of GAPDH?GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, evencritical,in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDHis a keyfactor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and actingas asignaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role intransporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407,11035021).Where in the cell is GAPDH localized?GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus(PMIDs: 21895736, 12829261).Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclearfunctions?Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression isuseful as a control for Western blots and RT-PCR.What are the known protein-protein interactions of GAPDH?GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some ofthese proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. Specification Tested Reactivity: human, mouse, rat, pig, zebrafish, yeast Cited Reactivity: human, rat, chicken, goat, yeast, deer, whiteflies, tick, treeshrew, cynomorium songaricum Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0766 Product name: Recombinant human GAPDH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC004109 Sequence: MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE Predict reactive species Full Name: glyceraldehyde-3-phosphate dehydrogenase Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC004109 Gene Symbol: GAPDH Gene ID (NCBI): 2597 RRID: AB_2107436 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04406 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.