Proteintech, 60006-1-Ig, CD38 Monoclonal antibody

Size: 20ul / 150ul The CD38 (60006-1-Ig) by Proteintech is a Monoclonal antibody targeting CD38 in WB, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse samples 60006-1-Ig targets CD38 in WB, IF/ICC, IF-P, ELISA, Cell treatment applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, Daudi cells, Jurkat cells, NK-92 cells, THP-1 cells, Ramos cells, Raji cells, TF-1 cells Positive IF-P detected in: mouse spleen tissue Positive IF/ICC detected in: Raji cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0487 Product name: Recombinant human CD38 protein Source: e coli. -derived, PKG Tag: GST Domain: 1-300 aa of BC007964 Sequence: MANCEFSPVSGDKPCCRLSRRAQLCLGVSILVLILVVVLAVVVPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI Predict reactive species Full Name: CD38 molecule Calculated Molecular Weight: 300 aa, 34 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC007964 Gene Symbol: CD38 Gene ID (NCBI): 952 ENSEMBL Gene ID: ENSG00000004468 RRID: AB_716994 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P28907 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.