Proteintech, 60042-1-Ig, CXCR4 Monoclonal antibody

Size: 20ul / 150ul The CXCR4 (60042-1-Ig) by Proteintech is a Monoclonal antibody targeting CXCR4 in WB, IHC, ELISA applications with reactivity to human samples 60042-1-Ig targets CXCR4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, Daudi cells, Ramos cells, THP-1 cells, Jurkat cells, Raji cells, K-562 cells Positive IHC detected in: human tonsillitis tissue, human breast cancer tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgM Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1528 Product name: Recombinant human CXCR4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 192-352 aa of BC020968 Sequence: NDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS Predict reactive species Full Name: chemokine (C-X-C motif) receptor 4 Calculated Molecular Weight: 352 aa, 40 kDa Observed Molecular Weight: 60~70 kDa GenBank Accession Number: BC020968 Gene Symbol: CXCR4 Gene ID (NCBI): 7852 RRID: AB_2091809 Conjugate: Unconjugated Form: Liquid Purification Method: Caprylic acid/ammonium sulfate precipitation UNIPROT ID: P61073 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.