Proteintech, 60065-1-Ig, CXCR3B-specific Monoclonal antibody

Size: 20ul / 150ul The CXCR3B-specific (60065-1-Ig) by Proteintech is a Monoclonal antibody targeting CXCR3B-specific in WB, IHC, ELISA applications with reactivity to human samples 60065-1-Ig targets CXCR3B-specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MOLT-4 cells, HT-29 cells, Jurkat cells, K-562 cells, U-937 cells Positive IHC detected in: human tonsillitis tissue, human liver cancer tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: chemokine (C-X-C motif) receptor 3 Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 43 kDa GenBank Accession Number: AF469635 Gene Symbol: CXCR3 Gene ID (NCBI): 2833 RRID: AB_2292376 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P49682-2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.