Proteintech, 60155-2-Ig, EPHA1-specific Monoclonal antibody

Size: 20ul / 150ul The EPHA1-specific (60155-2-Ig) by Proteintech is a Monoclonal antibody targeting EPHA1-specific in WB, IHC, ELISA applications with reactivity to human samples 60155-2-Ig targets EPHA1-specific in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, COLO 320 cells, HepG2 cells Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: EPH receptor A1 Calculated Molecular Weight: 108 kDa Observed Molecular Weight: 108 kDa GenBank Accession Number: BC130291 Gene Symbol: EPHA1 Gene ID (NCBI): 2041 RRID: AB_10646478 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P21709 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.