Proteintech, 60314-1-Ig, CREST Monoclonal antibody

Size: 20ul / 150ul The CREST (60314-1-Ig) by Proteintech is a Monoclonal antibody targeting CREST in WB, ELISA applications with reactivity to human, rat, mouse samples 60314-1-Ig targets CREST in WB, ELISA applications and shows reactivity with human, rat, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, COLO 320 cells, HSC-T6 cells, RAW 264.7 cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). Specification Tested Reactivity: human, rat, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag3119 Product name: Recombinant human CREST protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 47-396 aa of BC034494 Sequence: CTQYQQILHRNLVYLATIADSNQNMQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQGSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTLPTTSMSISGPGYSHAGPASQGVPMQGQGTIGNYVSRTNINMQSNPVSMIQQQAATSHYSSAQGGSQHYQGQSSIAMMGQGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGHGDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPSQQSYPGQQQGYGSAQGAPSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYGNYQQ Predict reactive species Full Name: synovial sarcoma translocation gene on chromosome 18-like 1 Calculated Molecular Weight: 396 aa, 43 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC034494 Gene Symbol: CREST Gene ID (NCBI): 26039 RRID: AB_2881425 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O75177 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.