Proteintech, 60316-1-Ig, VCP Monoclonal antibody

Size: 20ul / 150ul The VCP (60316-1-Ig) by Proteintech is a Monoclonal antibody targeting VCP in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 60316-1-Ig targets VCP in WB, IHC, IF/ICC, FC (Intra), ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: RAW 264.7 cells, WT and VCP KO U2OS cells, SH-SY5Y cells, HeLa cells Positive IHC detected in: human colon cancer tissue, human lung cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human stomach cancer tissue, mouse brain tissue, mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, SH-SY5Y cells Positive FC (Intra) detected in: HL-60 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975). Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1002 Product name: Recombinant human VCP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 345-644 aa of BC007562 Sequence: GMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG Predict reactive species Full Name: valosin-containing protein Calculated Molecular Weight: 89 kDa Observed Molecular Weight: 89 kDa GenBank Accession Number: BC007562 Gene Symbol: VCP Gene ID (NCBI): 7415 RRID: AB_2881427 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P55072 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.