Proteintech, 60335-1-Ig, E-cadherin Monoclonal antibody

Size: 20ul / 150ul The E-cadherin (60335-1-Ig) by Proteintech is a Monoclonal antibody targeting E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications with reactivity to human, mouse, rat, pig samples 60335-1-Ig targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: PC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells Positive IHC detected in: human breast cancer tissue, human colon tissue, rat colon tissue, rat stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human breast cancer tissue, human kidney tissue, MCF-7 cells Positive IF-Fro detected in: mouse brain tissue Positive IF/ICC detected in: MCF-7 cells, mouse breast cancer, HaCaT cells Recommended dilution Western Blot (WB): WB : 1:2000-1:8000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat, pig, canine, monkey Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag15085 Product name: Recombinant human E-cadherin protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 373-622 aa of BC141838 Sequence: PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPI Predict reactive species Full Name: cadherin 1, type 1, E-cadherin (epithelial) Calculated Molecular Weight: 882 aa, 97 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC141838 Gene Symbol: E-cadherin Gene ID (NCBI): 999 RRID: AB_2881444 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P12830 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.