Proteintech, 60342-1-Ig, APP Monoclonal antibody

Size: 20ul / 150ul The APP (60342-1-Ig) by Proteintech is a Monoclonal antibody targeting APP in WB, IHC, ELISA applications with reactivity to human, rat, pig, rabbit samples 60342-1-Ig targets APP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat, pig, rabbit samples. Tested Applications Positive WB detected in: pig brain tissue, HEK-293 cells, Y79 cells, rabbit brain tissue, rat brain tissue Positive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Specification Tested Reactivity: human, rat, pig, rabbit Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0769 Product name: Recombinant human APP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC004369 Sequence: MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREKWYKEVHSGQARWLML Predict reactive species Full Name: amyloid beta (A4) precursor protein Calculated Molecular Weight: 87 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC004369 Gene Symbol: APP Gene ID (NCBI): 351 RRID: AB_2881451 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P05067 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.