Proteintech, 60357-1-Ig, Myocilin Monoclonal antibody

Size: 20ul / 150ul The Myocilin (60357-1-Ig) by Proteintech is a Monoclonal antibody targeting Myocilin in WB, IHC, ELISA applications with reactivity to human, mouse, pig, rat samples 60357-1-Ig targets Myocilin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples. Tested Applications Positive WB detected in: pig heart tissue, human skeletal muscle tissue, human spleen tissue, rat heart tissue Positive IHC detected in: human heart tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). Specification Tested Reactivity: human, mouse, pig, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5492 Product name: Recombinant human MYOC protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 154-504 aa of BC029261 Sequence: EEKKRLRQENENLARRLESSSQEVARLRRGQCPQTRDTARAVPPGSREVSTWNLDTLAFQELKSELTEVPASRILKESPSGYLRSGEGDTGCGELVWVGEPLTLRTAETITGKYGVWMRDPKPTYPYTQETTWRIDTVGTDVRQVFEYDLISQFMQGYPSKVHILPRPLESTGAVVYSGSLYFQGAESRTVIRYELNTETVKAEKEIPGAGYHGQFPYSWGGYTDIDLAVDEAGLWVIYSTDEAKGAIVLSKLNPENLELEQTWETNIRKQSVANAFIICGTLYTVSSYTSADATVNFAYDTGTGISKTLTIPFKNRYKYSSMIDYNPLEKKLFAWDNLNMVTYDIKLSKM Predict reactive species Full Name: myocilin, trabecular meshwork inducible glucocorticoid response Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 50-57 kDa GenBank Accession Number: BC029261 Gene Symbol: Myocilin Gene ID (NCBI): 4653 RRID: AB_2881465 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99972 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.