Proteintech, 60562-4-Ig, PTRF Monoclonal antibody

Size: 20ul / 150ul The PTRF (60562-4-Ig) by Proteintech is a Monoclonal antibody targeting PTRF in WB, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 60562-4-Ig targets PTRF in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: HaCaT cells, A431 cells, A549 cells, HeLa cells, K-562 cells, rabbit colon tissue, rat colon tissue, mouse colon tissue, pig adipose tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transcription of RNA Pol I, which involves pausing of transcription by TTF1, and dissociation of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55 kDa. (PMID: 11139612 ) Specification Tested Reactivity: human, mouse, rat, pig, rabbit Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13495 Product name: Recombinant human PTRF protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-198 aa of BC008849 Sequence: MEDAAALELSSDEAVEVEEVIEESRAERIKRSGLRRVDDFKKAFSKEKMEKTKVRTRENLEKTRLKTKENLEKTRHTLEKRMNKLGTRLVPAERREKLKTSRDKLRKSFTPDHVVYARSKTAVYKVPPFTFHVKKIREGQVEVLKATEMVEVGADDDEGGAERGEAGDLRRGSSPDVHALLEITEESDAVLVDKSDSD Predict reactive species Full Name: polymerase I and transcript release factor Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC008849 Gene Symbol: PTRF Gene ID (NCBI): 284119 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6NZI2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.