Proteintech, 65072-1-Ig, Anti-Mouse CD45.2 (104)

Size: 500ug The CD45 65072-1-Ig targets CD45.2 in FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: mouse splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673). Specification Tested Reactivity: mouse Host / Isotype: Mouse / IgG2a, kappa Class: Monoclonal Type: Antibody Immunogen: B10.S mouse thymocytes and splenocytes Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, C GenBank Accession Number: BC028512 Gene Symbol: CD45 Gene ID (NCBI): 19264 RRID: AB_2918378 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P06800 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.