Proteintech, 65165-1-Ig, Anti-Human CD86 (BU63)

Size: 100ug / 500ug The CD86 (65165-1-Ig) by Proteintech is a Monoclonal antibody targeting CD86 in IF/ICC, FC applications with reactivity to human samples 65165-1-Ig targets CD86 in IF/ICC, FC applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: Raji cells Positive FC detected in: Daudi cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: B-lymphoblastoid cell line ARH 77 Predict reactive species Full Name: CD86 molecule Calculated Molecular Weight: 329 aa, 38 kDa GenBank Accession Number: BC040261 Gene Symbol: CD86 Gene ID (NCBI): 942 ENSEMBL Gene ID: ENSG00000114013 RRID: AB_2881481 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P42081 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.