Proteintech, 65181-1-Ig, Anti-Human CD146/MCAM (P1H12)

Size: 100ug / 500ug The CD146/MCAM (65181-1-Ig) by Proteintech is a Monoclonal antibody targeting CD146/MCAM in IF/ICC, FC applications with reactivity to human, mouse, rabbit, canine samples 65181-1-Ig targets CD146/MCAM in WB, IF/ICC, FC applications and shows reactivity with human, mouse, rabbit, canine samples. Tested Applications Positive IF/ICC detected in: HUVEC cells Positive FC detected in: HeLa cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). Specification Tested Reactivity: human, mouse, rabbit, canine Cited Reactivity: human Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: cultured human umbilical cells Predict reactive species Full Name: melanoma cell adhesion molecule Calculated Molecular Weight: 646 aa, 72 kDa GenBank Accession Number: BC056418 Gene Symbol: CD146 Gene ID (NCBI): 4162 RRID: AB_2881483 Conjugate: Unconjugated Form: Liquid Purification Method: Purified by protein-A affinity chromatography UNIPROT ID: P43121 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.