Proteintech, 65229-1-Ig, Anti-Rat CD11b/c (OX42)

Size: 100ug / 500ug The CD11b/c (65229-1-Ig) by Proteintech is a Monoclonal antibody targeting CD11b/c in FC applications with reactivity to rat samples 65229-1-Ig targets CD11b/c in FC applications and shows reactivity with rat samples. Tested Applications Positive FC detected in: LOU rat splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218). Specification Tested Reactivity: rat Host / Isotype: Mouse / IgG2a, kappa Class: Monoclonal Type: Antibody Immunogen: Rat peritoneal macrophages Predict reactive species Full Name: integrin alpha M Calculated Molecular Weight: 127 kDa GenBank Accession Number: NM_012711 Gene Symbol: CD11b Gene ID (NCBI): 25021 RRID: AB_3084908 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: Q9JI30 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.