Proteintech, 65236-1-Ig, CD71 Monoclonal antibody

Size: 20ul / 150ul The CD71 (65236-1-Ig) by Proteintech is a Monoclonal antibody targeting CD71 in WB, IHC applications with reactivity to human, mouse samples 65236-1-Ig targets CD71 in WB, IHC applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: PC-3 cells, RAW 264.7 cells, HeLa cells, K-562 cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: transferrin receptor (p90, CD71) Calculated Molecular Weight: 85 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC001188 Gene Symbol: CD71 Gene ID (NCBI): 7037 ENSEMBL Gene ID: ENSG00000072274 RRID: AB_3085030 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P02786 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.