Proteintech, 65239-1-Ig, Polyglutamine Monoclonal antibody

Size: 20ul / 150ul The Polyglutamine (65239-1-Ig) by Proteintech is a Monoclonal antibody targeting Polyglutamine in WB, ELISA applications with reactivity to n/a samples 65239-1-Ig targets Polyglutamine in WB, ELISA applications and shows reactivity with n/a samples. Tested Applications Positive WB detected in: Recombinant protein Positive ELISA detected in: Recombinant protein Recommended dilution Western Blot (WB): WB : 1:500-1:4000 Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:1000-1:20000 Background Information Huntington's disease is a neurodegenerative disorder caused by the expansion of a polyglutamine (polyQ) repeat in the N-terminal portion of huntingtin protein to a length above 35-40 units (PMID: 26047735; 19507258). The mutational expansion of polyglutamine above a critical length causes a toxic gain of function in huntingtin and results in neuronal death. In the course of the disease, expanded huntingtin is proteolyzed, becomes abnormally folded, and accumulates in oligomers, fibrils, and microscopic inclusions (PMID: 25336039). The anti-polyglutamine (polyQ) antibody MW1 specifically binds the polyQ domain of huntingtin exon 1. On western blot, the MW1 clone strongly prefers to bind to the expanded polyQ repeat form of Htt, displaying no detectable binding to normal huntingtin (PMID: 11719267). Specification Tested Reactivity: n/a Host / Isotype: Mouse / IgG2b, kappa Class: Monoclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: Polyglutamine GenBank Accession Number: N/A RRID: AB_3085031 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.