Proteintech, 65561-1-MR, Anti-Human CD163 (GHI/61) Mouse IgG2a Recombinant Antibody

Size: 100ug The CD163 (65561-1-MR) by Proteintech is a Recombinant antibody targeting CD163 in FC applications with reactivity to human samples 65561-1-MR targets CD163 in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: human PBMCs Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension Background Information CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164). Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Recombinant Type: Antibody Immunogen: Glycoprotein preparation from hairy cell leukemia spleen Predict reactive species Full Name: CD163 molecule Calculated Molecular Weight: 1156 aa, 125 kDa GenBank Accession Number: BC051281 Gene Symbol: CD163 Gene ID (NCBI): 9332 ENSEMBL Gene ID: ENSG00000177575 RRID: AB_3670315 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q86VB7 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.