Proteintech, 65562-1-MR, Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody

Size: 100ug The CD19 (65562-1-MR) by Proteintech is a Recombinant antibody targeting CD19 in FC applications with reactivity to human samples 65562-1-MR targets CD19 in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: human PBMCs Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908). Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Recombinant Type: Antibody Immunogen: Human chronic lymphocytic leukemia (CLL) cells Predict reactive species Full Name: CD19 molecule Calculated Molecular Weight: 556 aa, 61 kDa GenBank Accession Number: BC006338 Gene Symbol: CD19 Gene ID (NCBI): 930 ENSEMBL Gene ID: ENSG00000177455 RRID: AB_3670316 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P15391 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.