Proteintech, 65577-1-MR, Anti-Rat CD8a (OX-8) Mouse IgG2a Recombinant Antibody

Size: 100ug The CD8a (65577-1-MR) by Proteintech is a Recombinant antibody targeting CD8a in FC applications with reactivity to rat samples 65577-1-MR targets CD8a in FC applications and shows reactivity with rat samples. Tested Applications Positive FC detected in: rat splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084). Specification Tested Reactivity: rat Host / Isotype: Mouse / IgG2a Class: Recombinant Type: Antibody Immunogen: High-molecular-weight rat thymocyte glycoproteins Predict reactive species Full Name: CD8a molecule GenBank Accession Number: BC088126 Gene Symbol: Cd8a Gene ID (NCBI): 24930 RRID: AB_3670330 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P07725 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.