Proteintech, 65580-1-MR, Anti-Rat CD80 (3H5) Mouse IgG2a Recombinant Antibody

Size: 100ug The CD80 (65580-1-MR) by Proteintech is a Recombinant antibody targeting CD80 in FC applications with reactivity to rat samples 65580-1-MR targets CD80 in FC applications and shows reactivity with rat samples. Tested Applications Positive FC detected in: LPS-stimulated rat splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension Background Information CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215) Specification Tested Reactivity: rat Host / Isotype: Mouse / IgG2a Class: Recombinant Type: Antibody Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line Predict reactive species Full Name: Cd80 molecule Calculated Molecular Weight: 36 kDa GenBank Accession Number: XM_017597885 Gene Symbol: CD80 Gene ID (NCBI): 25408 RRID: AB_3670332 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: G3V671 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.