Proteintech, 65581-1-MR, Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody

Size: 100ug The CD83 (65581-1-MR) by Proteintech is a Recombinant antibody targeting CD83 in IF/ICC, FC applications with reactivity to human, non-human primates samples 65581-1-MR targets CD83 in IF/ICC, FC applications and shows reactivity with human, non-human primates samples. Tested Applications Positive IF/ICC detected in: Raji cells Positive FC detected in: Human monocyte-derived mature dendritic cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension Background Information CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928). Specification Tested Reactivity: human, non-human primates Host / Isotype: Mouse / IgG2a Class: Recombinant Type: Antibody Immunogen: Human CD83-transfected Cos cells Predict reactive species Full Name: CD83 molecule Calculated Molecular Weight: 205 aa, 23 kDa GenBank Accession Number: BC030830 Gene Symbol: CD83 Gene ID (NCBI): 9308 ENSEMBL Gene ID: ENSG00000112149 RRID: AB_3670333 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q01151 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.