Proteintech, 65586-1-MR, Anti-Human PD-1/CD279 (EH12.2H7) Mouse IgG2a Recombinant Antibody

Size: 100ug The PD-1/CD279 (65586-1-MR) by Proteintech is a Recombinant antibody targeting PD-1/CD279 in FC applications with reactivity to human samples 65586-1-MR targets PD-1/CD279 in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: PHA treated human PBMCs Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension Background Information Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Recombinant Type: Antibody Immunogen: N/A Predict reactive species Full Name: programmed cell death 1 Calculated Molecular Weight: 288 aa, 32 kDa GenBank Accession Number: BC074740 Gene Symbol: PD-1 Gene ID (NCBI): 5133 RRID: AB_3670336 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15116 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.