Proteintech, 65611-1-MR, Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Antibody

Size: 100ug The ALCAM (65611-1-MR) by Proteintech is a Recombinant antibody targeting ALCAM in IF/ICC, FC applications with reactivity to human samples 65611-1-MR targets ALCAM in IF/ICC, FC applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: hTERT-RPE1 cells Positive FC detected in: PHA treated human PBMCs Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension Background Information Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759). Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Recombinant Type: Antibody Immunogen: Cultured human thymic epithelial cells Predict reactive species Full Name: activated leukocyte cell adhesion molecule Calculated Molecular Weight: 105 kDa GenBank Accession Number: BC057809 Gene Symbol: ALCAM Gene ID (NCBI): 214 RRID: AB_3670358 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13740 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.