Proteintech, 65663-1-TR, Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rat Recombinant Antibody

Size: 100ug The MHC Class II (I-A/I-E) (65663-1-TR) by Proteintech is a Recombinant antibody targeting MHC Class II (I-A/I-E) in FC applications with reactivity to mouse samples 65663-1-TR targets MHC Class II (I-A/I-E) in FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: mouse splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 antibody is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436). Specification Tested Reactivity: mouse Host / Isotype: Rat / IgG2a Class: Recombinant Type: Antibody Immunogen: N/A Predict reactive species Full Name: histocompatibility 2, class II antigen A, alpha GenBank Accession Number: BC031711 Gene Symbol: MHC Class II Gene ID (NCBI): 14960 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P14434 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.